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Bio 231 Microbiology Lab Manual

HEAL100 Health Care — exam and revision material · Other

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Health Care exam material, 88 pages. Originally published on StudyMerit.

Pages
88
Academic year
2025/2026
Language
English

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Microbiology Laboratory Manual BIO231L Written by: Donna Cain, Hershell Hanks, Donna Cain, Hershell Hanks, Mary Weis, Mary Weis, Carroll Bottoms Carroll Bottoms, and, and Jonathan Lawson Jonathan Lawson Revised by: Tara Solgi 1 Downloaded by Clement Sam (samuelkaruiki40@gmail.com) lOMoARcPSD|6879242

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Table of Contents Experiment # Title Page - Biosafety Guidelines 3 - Microorganisms/Biosafety Levels 9 - Aseptic Technique 10 1 Microscopy 12 -- Staining of Bacterial Specimens 17 -- Preparing a Smear 19 2 Gram Stain 21 3 Capsule Stain 24 4 Endospore Stain 28 5 Culture Transfer Techniques 32 6 Isolation of Pure Cultures 35 7 Viable Plate Counts 39 8 Effect of Temperature on Microbial Growth 43 9 Atmospheric Oxygen Requirements 48 10 Use of Selective, Differential, and Enriched Media 52 11 Chemical Control of Microorganisms 60 12 Chemotherapeutic Agents 64 13 Additive and Synergistic Effects of Antibiotics 69 14 Microbiota of the Mouth 72 15 Normal Microbiota of the Throat and Skin 76 16 Yogurt Production 81 2 Downloaded by Clement Sam (samuelkaruiki40@gmail.com) lOMoARcPSD|6879242

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Learning Objectives After completing this lab, you should be able to:  Describe biosafety levels and identify several types of microorganisms under different BSL levels  Apply general laboratory safety rules in microbiology  Apply the aseptic techniques in microbiology laboratory  Properly use and care of a microscope Overview 1-1 Biosafety Guidelines 1-2 Biosafety Rules 1-3 Microorganisms and Biosafety Level 1-4 Aseptic Technique 1-5 M icroscopy Introduction In addition to following general laboratory safety rules, additional rules must be implemented in the microbiology lab since students are working with living organisms and the risk of student exposure to these organisms must be minimized. The four basic routes of exposure to microorganisms are (1) contact with skin and mucous membranes, (2) ingestion, (3) inhalation, and (4) inoculation. Specific lab safety guidelines are designed to address each of these potential routes of exposure. Contact with skin and mucous membranes can be minimized by wearing proper personal protective equipment such as lab coats or aprons, gloves, goggles, respirators, and face shields. In addition, students should be prohibited from inserting contact lenses in the lab. Ingestion of microorganisms can be minimized by prohibiting eating, drinking, or applying cosmetics in the lab. Inhalation of microorganisms can be minimized by adopting measures which decrease the likelihood of generating aerosols. Inoculation can be minimized by instituting rigid protocols for the use and disposal of sharps (needles, slides, broken glass, etc.) Microorganisms are divided into 4 Biosafety Levels (BSL) by the Centers for Disease Control and Prevention. The microbes used in our micro lab fall into the BSL-1 and BSL-2 categories. 3 Unit I Biosafety, Microorganisms and Microscopy 1-1: Biosafety Guidelines Downloaded by Clement Sam (samuelkaruiki40@gmail.com) lOMoARcPSD|6879242

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