Genetics: A Conceptual Approach, 5th Edition Test Bank

Test Bank for Chapter 2:Chromosomes and Cellular Reproduction Multiple-Choice Questions 1. Which of the following statements is false? a. b. c. d. e. Errors in chromosome separation are rarely a problem for an organism. Errors in chromosome separation can result in a miscarriage. Errors in chromosome separation can result in cancer. Errors in chromosome separation can result in a child with severe handicaps. Errors in chromosome separation can cause numerous problems for an organism. Answer: a Section: Introduction Comprehension 2. Which of these are NOT prokaryotes? a. b. c. d. Eubacteria Archaea Viruses Ancient bacteria Answer: c Section 2.1 Comprehension 3. Which statement is true? a. b. c. d. e. Eubacteria are more closely related to archaea than they are to eukaryotes. Archaea are more closely related to eukaryotes than they are to eubacteria. Eukaryotes are more closely related to eubacteria than they are to archaea. Viruses are more closely related to prokaryotes than they are to eukaryotes. Eubacteria, archaea, and eukaryotes are all equally related. Answer: b Section 2.1 Comprehension 4. Which of the following statements is false? a. b. c. d. Generally, chromosomes of prokaryotes are circular. Prokaryotes usually have a single molecule of DNA. Generally, chromosomes of eukaryotes are circular. Eukaryotes usually have multiple chromosomes. e. Eukaryote chromosomes are usually linear. Answer: c Section 2.1 Comprehension 5. In eukaryotes, chromosomes do not contain a. b. c. d. e. ribosomes. chromatin. proteins. histones. DNA. Answer: a Section 2.1 Comprehension 6. Prokaryotic chromosomes do not have telomeres because they a. b. c. d. e. do not go through mitosis. do not go through DNA replication. are in the cytoplasm. are circular. have no centromeres. Answer: d Section 2.1-2.2 Comprehension 7. In prokaryotes, replication usually begins at a specific place on the chromosome called the a. b. c. d. e. binary fission site. origin of replication. origin of mitosis. anchoring site. kinetochore. Answer: b Section 2.2 Comprehension 8. The highly organized internal scaffolding of the nucleus is called the a. histone complex. b. spindle microtubules. c. nuclear cohesion. d. nuclear matrix. e. nuclear envelope. Answer: d Section 2.2 Comprehension 9. The attachment point on the chromosome for spindle microtubules is the a. b. c. d. e. telomere. centromere. origin of replication. sister chromatid. allele. Answer: b Section 2.2 Comprehension 10. The process of splitting the cytoplasm, which separates one cell into two, is termed a. b. c. d. e. cytokinesis. mitosis. anaphase. diakinesis. fusion. Answer: a Section 2.2 Comprehension 11. In order to be functional, a eukaryotic chromosome requires all of the following a. b. c. d. a centromere. origins of replication. a plasmid. telomeres. Answer: c Section 2.2 Comprehension 12. Diploid cells are cells with a. a single set of chromosomes. b. circular chromosomes. c. two sets of chromosomes. except d. many sets of chromosomes. e. three sets of chromosomes Answer: c Section 2.2 Comprehension 13. If a healthy cell passes the G1/S checkpoint, a. b. c. d. e. it will enter the G0 stage of the cell cycle. DNA will be replicated. it will not divide. it will proceed immediately to cytokinesis. it will die. Answer: b Section 2.2 Comprehension 14. Which of the following does NOT occur during the G2 phase of the cell cycle? a. b. c. d. The G2/M checkpoint is reached. DNA replication and error checking is completed. The cell completes preparation for mitosis. The cell divides. Answer: d Section 2.2 Comprehension 15. Which of the following occurs during prometaphase? a. b. c. d. e. The chromosomes align in a single plane. DNA is replicated. Microtubules attach to the kinetochores. Mitotic spindles form. The two sister chromatids separate. Answer: c Section 2.2 Comprehension 16. Chromosome movement during anaphase is a result of a. disassembly of tubulin molecules by molecular motor proteins. b. kinetochore shortening causing chromosomes to pull apart. c. metaphasal plate splitting resulting in chromosomal disassembly. d. the cohesion protein attaching to the centromeres of sister chromatids. e. cilia movement inside the cellular structure. Answer: a Section 2.2 Comprehension 17. Pea plants have seven different types of chromosomes. A chromosome with a centromere at the very end is called a. b. c. d. e. submetacentric. metacentric. acrocentric. acentric. telocentric. Answer: e Section 2.2 Comprehension 18. A dividing eukaryotic cell is treated with a drug that inhibits the molecular motors associated with kinetochores. At which cell cycle stage would it stop? a. b. c. d. e. G1 S G2 M (metaphase) M (telophase) Answer: d Section 2.2 Application 19. Pea plants have seven different types of chromosomes. The nucleus of a megaspore in a pea ovary would contain how many chromosomes? a. b. c. d. e. 3½ 7 14 21 30 Answer: b Section 2.3 Comprehension 20. Pea plants have seven different types of chromosomes. A nucleus in the pea endosperm contains how many chromosomes? a. b. c. d. e. 3½ 7 14 21 30 Answer: d Section 2.3 Comprehension 21. What process is unique to plants? a. b. c. d. e. Meiosis Double fertilization Crossing over Haploid gametes Spermatogenesis Answer: b Section 2.3 Comprehension 22. Suppose that a diploid cell contains 8 chromosomes (2n = 8). How many different combinations in the gametes are possible? a. b. c. d. e. 2 4 8 16 64 Answer: d Section 2.3 Comprehension 23. A diploid somatic cell from a rat has a total of 42 chromosomes (2n = 42). As in 5.The figure shows a chromosomal separation taking place. The letters stand for genes; capital and lowercase stand for different alleles. The diploid chromosome number in this organism is four.What process is shown? B A A a. b. c. d. e. b C C d d Anaphase of mitosis Telophase of meiosis I Anaphase of meiosis I Telophase of mitosis Anaphase of meiosis II Answer: e Section 2.3 Comprehension 24. In a flowering plant, the male part of the flower (the stamen) produces haploid microspores that divide by _____to produce sperm. a. b. c. d. e. mitosis meiosis gametogenesis spermatogenesis fertilization Answer: a Section 2.3 Comprehension 25. A pollen grain that lands on a stigma grows a pollen tube to deliver ___ (how many?) sperm to the ovary. Fusion of a sperm with an egg produces a ___n cell called a___. a. 1; 1; zygote b. 2; 1; megasporocyte c. 2; 2; zygote d. 1; 2; microsporocyte e. 1; 2; megasporocyte Answer: c Section 2.3 Comprehension 26. To provide food for the developing embryo, a tissue called endosperm is produced through double fertilization. Endosperm has a ploidy of a. b. c. d. e. 1n. 2n. 3n. 4n. 5n. Answer: c Section 2.3 Comprehension 27. What might be the result if breakdown of the shugoshin protein were premature? a. b. c. d. e. The cohesion protein would hold the chromosome arms together longer. The separation of homologous chromosomes would occur prematurely. The separation of sister chromatids would occur prematurely. Spindle fibers wouldn’t form. Sister chromatids would never separate. Answer: c Section 2.3 Comprehension 28. A diploid somatic cell from a rat has a total of 42 chromosomes (2n = 42). As inhumans, sex chromosomes determine sex: XX in females and XY in males. What isthe total number of telomeres in a rat cell in G2? a. b. c. d. e. 21 42 84 126 168 Answer: e Section 2.3 Application 29. A diploid somatic cell from a rat has a total of 42 chromosomes (2n = 42). As in humans, sex chromosomes determine sex: XX in females and XY in males. What is the total number of chromosomes present in the cell during metaphase I of meiosis? a. b. c. d. e. 21 42 84 126 168 Answer: b Section 2.3 Application 30. A diploid somatic cell from a rat has a total of 42 chromosomes (2n = 42). As in humans, sex chromosomes determine sex: XX in females and XY in males. What is the total number of chromosomes in a polar body cell from a rat? a. b. c. d. e. 21 40 41 42 84 Answer: a Section 2.3 Application Short-Answer Questions 31. What evidence is there that viruses evolved after, not before, cells? Answer: Viruses can reproduce only within host cells. Thus, they must have evolved after cells. Section 2.1 Comprehension 32. Why is mitosis important within the cell cycle? Answer:A single cell and all its genetic information is duplicated. Each cell contains a full complement of chromosomes. Section 2.2 Comprehension 33. Explain why mitosis does not produce genetic variation and how meiosis leads to the production of tremendous genetic variation. Answer:Mitosis produces cells that are genetically identical to the parent cell. Meiosis includes two distinct processes that contribute to the generation of genetic variation: Crossing over shuffles alleles on the same chromosome into new combinations, whereas the random distribution of maternal and paternal chromosomes shuffles alleles on different chromosomes into new combinations. Section 2.2 Comprehension 34. Microscopy to look at a cell’s chromosomes is often done when the cell is in mitotic metaphase. For example, karyotypes that extract chromosomes from a single cell and photograph them to look for abnormalities are done on metaphase, rather than interphase, cells. Why? Answer: In metaphase, chromosomes are condensed and are more easily visualized. Section 2.2 Application 35. List and briefly describe the three major cell cycle checkpoints. For each checkpoint, predict the consequences if the checkpoint failed to work properly. Answer: (1) The G1/S checkpoint holds the cell in G1 until the cell has all of the enzymesnecessary for replication of DNA. If the checkpoint failed, the cell would proceed into S without the necessary enzymes, causing the DNA not to be replicated properly or completely. This might cause the cell cycle to halt at the G2/M checkpoint. Alternatively, the cell might divide without the genetic material having been replicated, causing the daughter cells to receive incomplete genetic information. (Both predictions are reasonable based on information in the chapter.) (2) The G2/M checkpoint is passed only if the cell’s DNA is undamaged. If it fails to work properly, division would proceed in the presence of damaged DNA, possibly leading to mutations in the daughter cells and/or death of the daughter cells. (3) The spindle-assembly checkpoint is during metaphase, and it ensures that each chromosome is aligned at the metaphase plate and attached to spindle fibers from opposite poles. This checkpoint depends on tension at the kinetochores of each chromosome. If the checkpoint fails, anaphase will occur even when the chromosomes are not aligned properly, allowing daughter cells to be produced with extra and/or missing chromosomes. Section 2.2 Application 36. Describe what is happening to chromosomes during the five substages of prophase I. Answer: Leptotene – chromosomes contract and become visible Zygotene – chromosomes continue to condense and homologous chromosomes pair up and begin synapsis Pachytene – chromosomes become shorter and thicker, synaptonemal complex develops between homologous chromosomes. Diplotene – centromeres of paired chromosomes move apart – the two homologs remain attached at each chiasma Diakinesis – centromeres have moved apart Section 2.2 Application 37. Describe the difference between the centromere and kinetochore. Answer:A centromere is the physical location on a chromosome where the kinetochore and spindle microtubules attach. The kinetochore is composed of proteins that assemble on the centromere to provide a site for the spindle microtubules to attach. Section 2.2 Application 38. Describe the difference between G1 and G2 of the cell cycle. Answer:G1 occurs before S phase and G2 occurs after S phase. During G1, cells grow in size, chromosomes are composed of a single chromatid. During G1, cells pass a critical checkpoint (the G1/S checkpoint) after which they are committed to undergoing cell division. During G2, the chromosomes are composed of two chromatids. There is another checkpoint during G2 that ensures cells are prepared for mitosis. Cells typically spend more time in G1 than in G2. Section 2.2 Application 39. (a) Draw a pair of telocentric homologous chromosomes as they would appear in G2. Indicate centromeres with a small circle, and place the alleles A and a on each of the chromatids. (b) Draw the same chromosomes as they would appear in G1. Place the alleles ―A‖ and ―a‖ on each of the chromatids. Answer: (a) A Section 2.2 (b) a a A a Application 40. In tissue from the intestinal epithelium of a frog, the following proportions of cells were found at each stage of the cell cycle: Stage Interphase Prophase Prometaphase Metaphase Anaphase Telophase Proportion of Cells 0.90 0.04 0.02 0.01 0.02 0.01 If the entire cell cycle in frog epithelium cells requires 20 hours for completion, what is the average duration of each stage? Answer:0.9 × 20 = 18 hours, 0.04 × 20 = 0.8 hours, 0.02 × 20 = 0.4 hours, etc. Section 2.2 Challenge 41. Find and describe at least four errors in the drawing below of mitotic anaphase. A- -A B- -b d- B- dD- D- Answer: -b (1) Chromosomes that are separating are still duplicated. (2) Spindles are not coming from a common spindle-pole body. (3) Sister chromatids do not have identical alleles for the B gene. (4) Two alleles of the D gene are on one chromosome. (5) No alleles of the A gene are on the homologous chromosome. (6) Homologous chromosomes appear to have paired and to be segregating. Section 2.2 Challenge 42. The cells illustrated below belong to a species with a diploid chromosome number of four. Each of the cells below is in which stage of mitosis or meiosis? a. b. c. d. e. Answer: a. Meiosis I metaphase b. Mitosis metaphase c. Mitosis anaphase d. Meiosis I anaphase e. Meiosis II metaphase Section 2.2-2.3 Comprehension 43. Using the following choices, indicate the correct phase(s) in parts a-e. 1. 2. 3. 4. 5. 6. Meiosis I prophase Meiosis I anaphase Meiosis II prophase Meiosis II anaphase Mitosis prophase Mitosis anaphase a. Chromosomes are in unseparated, sister-chromatid form, at the end of the phase(s) _____. b. The first stage after which a dividing cell that started as a diploid would be haploid. c. Sister chromatids separate during _____. d. Chromosomes are randomly partitioned during _____, contributing to genetic diversity. e. Crossing over (genetic recombination) occurs in _____. Answer: a. 1, 2, 3, 5 b. 2 c. 4, 6 d. 2 e. 1 Section 2.2-2.3 Comprehension 44. During prophase I of meiosis, crossing over is indicated by what microscopically visible structure? Answer:Chiasmata (chiasma) or the synaptonemal complex Section 2.3 Comprehension 45. What is one feature of meiosis that produces genetic variability in gametes? In two or three sentences, explain how this feature causes genetic uniqueness. Answer: (1) Independent assortment.In meiosis I—metaphase and anaphase—nonhomologous chromosomes distribute randomly. Alignment and separation of one pair of homologous chromosomes is independent of how a different pair separates. Different gametes have different chromosomes can have different alleles for the same genes, so the gametes normally have different combinations of alleles. (2) Crossing over.In meiosis I—prophase—portions of homologous chromosomes exchange, changing combinations of alleles of genes on a single chromosome, so not even sister chromatids are identical after crossing over. Each gamete has only one copy of each homolog, and each homolog now has a unique combination of alleles. Section 2.3 Comprehension 46. List two differences and two similarities between mitosis and meiosis. Answer: Differences: (1) Mitosis occurs in somatic (nonsex) cells; meiosis occurs in sex cells to produce gametes. (2) Meiosis involves chromosome pairing (of homologous chromosomes); mitosis does not. (3) Mitosis produces nonsex cells; meiosis produces gametes. (4) Mitosis produces cells of the same ploidy; meiosis produces haploid cells from diploid cells. (5) Meiosis has two consecutive divisions; mitosis has one. (6) Mitosis produces two daughter cells; meiosis produces four daughter cells. (7) Mitosis produces identical daughter cells; meiosis produces four different daughter cells. Similarities: (1) Both involve the separation of replicated chromosomes during cell division. (2) Both are processes to ensure that daughter cells in cell division receive a complete set of chromosomes. (3) DNA replication must occur first. (4) Cytokinesis usually occurs at the end of each. Section 2.2-2.3 Application 47. Describe the difference between homologous chromosomes and sister chromatids. Answer:Homologous chromosomes can have different alleles. Sister chromatids are duplicates and (except for errors in replication) are identical in sequence. Section 2.3 Application 48. Describe the difference between meiosis I and meiosis II. Answer:Homologs pair and segregate in meiosis I. Sister chromatids are paired and segregate in meiosis II. Crossing over occurs in meiosis I but not in meiosis II. Section 2.3 Application 49. Describe the difference between the sporophyte and gametophyte. Answer:The sporophyte is the diploid phase of a plant life cycle. The gametophyte is the haploid stage. Section 2.3 Application 50. What events during sexual reproduction are significant in contributing to genetic diversity? Answer: (1) Crossing over changes allele combinations on chromosomes, so, after meiosis I, even sister chromatids are not genetically identical. (2) Independent assortment of non-homologous chromosomes ensures each gamete has a different combination of alleles for genes on non-homologs. (3) Two genetically unique gametes from each parent combine during fertilization to form a novel, genetically unique individual. Section 2.3 Application 51. Write all possible genotypes of each of the cells resulting from mitosis and meiosis, drawn in the previous question. Answer: Mitosis: A/a B/b D/ior ABD/abd (diploid and heterozygous at all three loci) Meiosis: ABd, aBd, AbD, abD (haploid at all three loci) Section 2.3 Application 52. A diploid, eukaryotic cell in interphase has these two pairs of homologous chromosomes with the indicated arrangement of alleles: A a D b d B Draw the chromosomes at the end of (a) prophase of mitosis and (b) prophase I (of meiosis I) with the most likely crossing over events. Indicate placement of alleles on the chromosomes. Answer: (a) A A b b a A a b a B B B d D d d D (b) A b a B A d b a D B d Section 2.3 Application 53. A diploid, eukaryotic cell in interphase has these two pairs of homologous chromosomes with the indicated arrangement of alleles A a D b d B Draw the chromosomes at the end of telophase of (a) mitosis and (b) meiosis II. Indicate placement of alleles on the chromosomes. Answer: (a) a A b A a d B b B d D D (b) [One possibility] a d B B d D a A b D b Section 2.3 Application 54. (a) Compare and contrast spermatogenesis and oogenesis in animals. For each process, be sure to include information about division of the nucleus, allocation of chromosomes to the various products, and division of the cytoplasm. (b) Why is the difference in cytoplasmic division between spermatogenesis and oogenesis important to reproduction, considering the different roles of sperm and eggs in reproduction? Answer: (a) Division of the nucleus and allocation of the chromosomes to the products are essentially the same in both processes. Starting with a 2n germ cell, nuclear division is by meiosis I and II, and each product of meiosis contains one set of chromosomes (1n). The major difference is that division of the cytoplasm during meiosis I and II is equal in spermatogenesis and unequal in oogenesis. During oogenesis, meiosis I produces a large secondary oocyte with lots of cytoplasm and a polar body with very little cytoplasm. Meiosis II in the secondary oocyte produces a large ovum with lots of cytoplasm and a small second polar body. Therefore, only one large, functional egg is produced per primary oocyte, whereas four small, functional sperm are normally produced per primary spermatocyte. (b) The small size and other features of sperm structure suit them well to delivery of the haploid nucleus to the egg. The large amount of cytoplasm in the egg suits it well to nourishing development of the embryo after fertilization. Section 2.3 Application 55. (a) Describe the changing role of cohesin during the mitotic cell cycle. (b) Explain the importance of regulation of cohesin activity to normal cell division. Answer: (a) Cohesin keeps sister chromatids together after DNA replication during S phase through metaphase of mitosis. The breakdown of cohesin allows the sister chromatids to separate from each other during anaphase. (b) Cohesin must be active beginning in S phase through metaphase in order to keep the sister chromatids together so that they can be properly aligned at the metaphase plate to ensure equal division of the genetic information to the two daughter cells. Cohesin must be inactivated or broken down in order to allow the sister chromatids to separate during anaphase so that each daughter cell will get one copy of the genes on each chromosome. Section 2.3 Application